Canopy light distribution effects on light use efficiency in wheat and its mechanism

Optimizing canopy light distribution (CLD) has manifested improved utility and yield without modifying other inputs. Nonetheless, molecular mechanisms working at cellular organelle level remain to be elucidated. The current study aimed assess the effect of CLD on photosynthetic performance wheat, investigate into mechanism underlying photosynthetically active radiation (PAR)–use efficiency (PUE) optimized CLD. Wheat was planted in two rows having different spacing [R1 (15 cm) R2 (25 cm)] simulate Flag penultimate leaves were subjected chloroplast proteomics analysis. An increase row positively affects A decrease (16.64%) PAR interception upper layer, an (19.76%) middle PUE (12.08%), increased (9.38%) recorded. abundance proteins associated with electron transport, redox state, carbon-nitrogen assimilation differentially altered by optimization. In leaves, reduced photosystem II (PSII) light-harvesting proteins, PSII-subunits, I (PSI) NAD(P)H quinone oxidoreductase (NQO) enzymes involved carbon compared R1. Additionally, leaf stomatal conductance increased. Altogether, these findings demonstrated that regulation proteome is intimately linked utilization, which provide basis for genetic manipulation crop species better adaptation improvement cultivation strategies.